Bone Marrow Transplantation With Specially Treated Bone Marrow in Treating Patients With Hematologic Cancer That Have Not Responded to Previous Therapy

OBJECTIVES: I. Determine if patients with refractory, high risk hematologic malignancies or bone marrow failure who receive HLA haploidentical bone marrow treated with anti-B7 antibody have normal engraftment. II. Determine if these patients are free of hyperacute graft versus host disease (GVHD), defined as grade D GVHD in the first 10 posttransplant days, when treated with this regimen. III. Determine if these patients have an acceptable incidence of life threatening grade D GHVD in the first 50 posttransplant days following this treatment regimen. IV. Determine the safety and tolerability of this treatment regimen in this patient population.

OUTLINE: This is a multicenter study. Patients undergo leukapheresis to collect white blood cells which are incubated with donor bone marrow cells in the presence of anti-B7.1 and anti-B7.2 antibodies for 36 hours. Patients receive total body irradiation twice daily on days -6 to -3, cyclophosphamide IV daily on days -2 and -1, and methylprednisolone IV every 12 hours for a total of 4 doses on days -2 to 0. Patients are infused with the treated donor bone marrow on day 0. Patients then receive methotrexate IV on days 1, 3, 6, and 11 and leucovorin calcium IV 24 hours after each dose of methotrexate every 6 hours for 3-8 doses each time. Patients also receive cyclosporine IV or orally twice daily on days -2 to 100. Patients are followed every 2 months for 1 year.

PROJECTED ACCRUAL: A total of 20 patients will be accrued for this study.

Inclusion criteria

• Age ≤40 years.
• Diagnoses-patients with the following hematologic malignancies and bone marrow failure syndromes:

• Acute myelogenous leukemia-induction failure, relapse, second or greater complete remission (CR)
• Acute lymphocytic leukemia-induction failure, relapse, second or greater CR, first CR with t(9;22), t(8;14), or t(4;11)
• Non-Hodgkin's lymphoma (intermediate or high grade) which has failed to achieve CR with at least two induction regimens, relapse, second or greater CR
• Multiple myeloma with poor prognostic features (elevated 0-2 microglobulin or high labeling index)
• Hodgkin's disease in relapse or which fails to achieve CR after two chemotherapy regimens
• Congenital or acquired bone marrow failure - poorly responsive to or intolerant of current therapy
• Myelodysplastic syndrome of all subtypes except refractory anemia (RA)
• Patient has a haploidentical family member that meets medical criteria for donation.
• Eligibility for other transplant types:

• Patient considered likely to have clinical deterioration and rapid disease progression during an unrelated donor search, or
• Patient who has already had an unproductive donor search or
• Patient ineligible for or has refused autologous transplant
• Adequate renal and hepatic function for age:

• Serum creatinine <2 x ULN
• Alanine aminotransferase (ALT, SGPT) x ULN
• Aspartate aminotransferase (AST, SGOT) x ULN
• Total bilirubin 5_2 x ULN except if bilirubin is elevated due to Gilbert's syndrome or hemolytic anemia
• Adequate cardiac and pulmonary function for age.
• ECOG Performance Status 0, 1, or 2 or Lansky performance scale >50% for patients <16 years of age.
• Voluntary witnessed written informed consent. Children will be asked for assent where appropriate.
• The patient, if female, must be post-menopausal, premenarcheal, or sterile, or if the patient is of childbearing potential, she must be practicing a method of birth control considered effective and medically acceptable by the investigator for a minimum of 1 month prior to study entry and at least 2 months after the study end.
• Patient must have undergone successful leukapheresis to obtain adequate antigen presenting cells.
• Any patient who enters the study in a relapse state, with evidence of end organ (pulmonary, renal, or hepatic) toxicity, or with recent recovery from infection, who may potentially have little benefit from this protocol, must have his/her eligibility status discussed with the Principal Investigator.
• Patient must have life expectancy of at least 12 weeks.

Exclusion criteria

• Eligibility for other transplant types:

• Patient has family donor who is matched or single antigen mismatched at HLA-A, HLA-B, HLA-DR, and HLA-DQ. Donorrecipient matching must be evaluated via both phenotype and genotype.
• Patient has available unrelated donor who is matched at HLA-A, HLA-B, and HLA-DR. Donor-recipient matching must be evaluated via both phenotype and genotype.
• Active uncontrolled infection (continued positive blood or soft tissue cultures despite appropriate antibiotic treatment)
• Positive 13-HCG in a female of childbearing potential
• Evidence of HIV infection or known HIV positive serology
• Any prior bone marrow transplant
• A peripheral blood differential count at the time of leukapheresis with greater than 25% blasts. This exclusion criterion is valid only for the first four patients enrolled.
• Patients with Fanconi's anemia