Skip to main content
OpenTrials
Recruiting

NCT Number: NCT07228598

Biofilm on Chest Drains After Thoracic Surgery: Clinical Impact and Antibiotic Resistance

This prospective observational study aims to investigate the frequency and clinical significance of biofilm formation on thoracic chest drains used after thoracic surgery. Biofilms are structured bacterial communities that adhere to surfaces and can promote infection and antibiotic resistance. In this study, small samples from the tips of chest drains will be collected under sterile conditions at the time of drain removal and analyzed in the microbiology laboratory.

The main objectives are to evaluate the relationship between the duration of chest drain placement and the presence of biofilm, and to identify the antibiotic resistance profiles of microorganisms isolated from these biofilms. Secondary outcomes include the association between biofilm formation, postoperative infections (such as empyema or wound infection), and the length of hospital stay.

This study will be conducted at Ondokuz Mayıs University Faculty of Medicine, Department of Thoracic Surgery, in collaboration with the Department of Microbiology. The findings are expected to contribute to better understanding of optimal drain management and infection prevention strategies after thoracic surgery.

Recruiting

Interested in participating?

Request Info

Key information

Age range

18 year and older

Sex eligibility

All sexes

Study type

Observational

Primary location

Ondokuz Mayis University Medical Faculty, Department of Thoracic Surgery

Samsun, 55139, Turkey (Türkiye)

Location status: Recruiting

Location contact

Caner İşevi, MD

CONTACT

[email protected]

03623121919 ext. 4240

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Age 18 years and older
  • Undergoing elective thoracic surgery requiring chest drain placement
  • Able and willing to provide written informed consent

Exclusion criteria

  • Presence of active infection prior to surgery
  • Emergency thoracic surgery
  • Immunosuppressed patients (e.g., neutropenia, advanced malignancy, organ transplantation)
  • Patients receiving systemic antibiotic therapy at the time of drain removal
  • Drainage for empyema or pre-existing pleural infection
  • Presence of extrathoracic infection focus
  • Refusal or inability to provide informed consent

Treatment and study plan

Chest Drain Tip Sampling

Other

No active intervention; routine chest drain removal followed by microbiological and biofilm analysis of drain tip samples.

Primary outcomes

  1. Presence and Intensity of Biofilm Formation on Chest Drain Tips

    Time frame: At the time of chest drain removal (postoperative days 1-7)

    Biofilm formation on the inner surface of chest drain tips will be evaluated using a microplate crystal violet staining method. The optical density (OD) at 492 nm will be measured, and biofilm intensity will be classified according to the Christensen and Chusri scale (non, weak, moderate, strong). The proportion of patients with biofilm-positive drain tips will be recorded and compared across drain duration groups.

Sponsors and collaborators

Lead sponsor

Caner İşevi, MD

Other

Registry information

Official study title

Biofilm Formation on Thoracic Drains: Clinical Significance and Association With Antibiotic Resistance

Important dates

Study start
2025
Primary completion
2026
Study completion
2026
First posted
Nov 14, 2025
Registry last updated
Jul 24, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.