Bioavailability of Different Vitamin K Vitamers Studied Using 13C-labelled Vitamin K Vitamers

Overall study design Pilot study (n= 2 completers): An acute postprandial randomised parallel study comparing the bioavailability of two forms of labelled vitamin K vitamers (13C-PK and 13C-MK-7) Study part I (n= 16 completers): An acute postprandial randomised cross-over study comparing the bioavailability of four forms of labelled vitamin K vitamers (13C-PK, 13C-MK-4, 13C-MK-7, and 13C-MK-9)

Detailed study design of pilot study and part I Prerequisites

Prior to the first test period (day 0) the participants will be asked to:

• 2 weeks before: supplement with 10 µg vitamin D3/day (recommended daily intake) and restrain from all other types of (vitamins and mineral) supplementation
• 1 week before: not consume sweet corns, beetroot and spinach
• 48 hours before: alcohol intake is prohibited
• 24 hours before: high-intensity physical activity is prohibited
• 12 hours before: except 0.5 L tap water, consumption of drinks and foods are not allowed After the first test day and during the remaining part of the study, the participants are asked to refrain from consumption of sweet corn, beetroot and spinach, continue supplementation with vitamin D. Before all days with blood sampling (3 first days in a test period), the above prerequisites related to alcohol intake, high-intensity physical activity and fasting are applicable.

Test periods A baseline description (e.g. anthropometry, blood pressure and fasting values) of the participants will be established prior to and on the first test day (Day 0).

In the pilot study, there will be one test period (Day 0-2) and in the study part I there will be four test periods (Day 0-2, 7-9, 14-15 and 21-23). Except for a few baseline outcomes only collected at Day 0, all test periods will follow the same overall design.

On the first day in a test period (e.g. Day 0), the participants will arrive fasting at NEXS, where a peripheral venous catheter (PVC) will be placed. The participants will rest for 10 minutes before the first blood sample will be taken in the fasting state ("0" minutes). The participants will hereafter have 15 minutes to ingest a breakfast meal containing a 13C-labelled vitamin K vitamer. Postprandial blood samples will hereafter be collected during a 5.5-hour period at the following time points after the breakfast was initiated: 15, 30, 45, 60, 90, 150, 210, 270 and 330 minutes. Furthermore, on the 2 consecutive days a fasting blood sample will be collected corresponding to 24 (±2) hours and 48 (±2) hours after the ingestion of the 13C-labelled vitamin K vitamer.

Results from the pilot study will be used to decide which biological sampling time points that are necessary in study part I where a maximum blood volume per test period will be 125 mL (for all 4 test periods the maximum blood volume will be 500 mL).

Wash out period Participants in the pilot study will have one washout period and participants in study part I will have 4 washout periods. During washout, the participants do not ingest the 13C-labelled vitamin K vitamer. In part I, the washout periods are minimum 6 days to ensure no carry over to the next administration period. Optimally, these washout periods will be planned at days: 1-6 (test period

1), 8-13 (test period 2), 15-20 (test period 3) and 22-27 (test period 4) and the participants will continue the collection of urine and faecal samples during the washout period. On day 7, participants in part I will repeat the test period (except the run-in period) and thus it continues until all 4 vitamin K vitamers have been tested.

In case of illness, vacations, holidays or practical project management reasons, the washout period can be extended. Extension of the washout period is not defined as a protocol deviation if all biological samples are collected on the consecutive days according to time points/definitions.

Inclusion criteria

• Men and women
• Between 18 and 65 years old (including both 18 and 65 year olds)
• Danish-speaking
• Willingness to consume/comply with consumption of study-related intervention products/diet
• Willing to take 10 µg vitamin D3/day and omit intake of all other (vitamins and mineral) supplements before and during the trial
• BMI ≤ 30.0 kg/m2

Exclusion criteria

• Daily smokers/users of all kinds of nicotine-containing products. Occasional smokers/user can be included if they are willing to refrain from all kinds of nicotine-containing products during the trial (from screening to end of trial)
• Blood donation <3 months prior to study-related blood sampling
• Intensive physical training (> 10 hours of strenuous physical activity per week)
• Participation in other clinical studies at the time of the study
• Pregnant, lactating or planning to become pregnant within the study period
• Habitual alcohol intake above current recommendation (> 10 alcoholic units/week)
• History of cancer within the past five (5) years except basal cell skin cancer and cervix
• Diagnosed with chronic inflammation disorders
• Diagnosed with gastrointestinal diseases
• Diagnosed with bone related diseases
• Diagnosed with psychiatric disorder including depression that requires medical treatment
• Medical treatment of diabetes
• Medical treatment of cardiovascular related disease
• Surgical change of the gastrointestinal tract (removal of appendix is allowed)
• Use of prescription medication that can affect their safety or impact on data
• Inability, physically or mental, to comply with the procedure required by the study protocol and evaluated by the principal investigator (PI), study manager or clinical responsible
• Medical treatment with anticoagulation medication (warfarin-like types)
• Allergy or intolerance related to intervention products