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Completed

NCT Number: NCT01743924

Bioavailability of Chemopreventive and Nutritional Compounds in Broccoli

The aim of this study was to determine the absorption of isothiocyanates (ITC) after ingestion of kailan-hybrid broccoli through the analysis of the correspondent urinary biomarkers. Furthermore, the effect of cooking (microwave) on the mentioned metabolic fate of these ITC was studied comparing to the uncooked vegetable.

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Key information

Conditions

Bioavailability

Age range

22 year–50 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Early Phase 1

Primary location

Hospital General Universitario Reina Sofía

Murcia, 30003, Spain

About this study

Background Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to ITC, which have chemopreventive properties. No information has been reported about the metabolic fate of ITC in the kailan-hybrid broccoli, which physicochemical characteristics could greatly influence that metabolic fate. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds.

Research Methods & Procedures A cross-over-design study of 7 subjects after ingestion of 200-g raw or microwaved kailan-hybrid broccoli was conducted. Total ITC equivalents in the vegetable, urine and blood samples were determined by HPLC as the cyclocondensation product of 1,2-benzenedithiol.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Subjects with a body mass index between 19 and 28.
  • Subjects age: 22-50 years-old.
  • Good health of subjects: confirmed by medical history, physical examination and clinical laboratory analysis of urine and blood (complete blood count and liver function markers including serum glutamic-pyruvic transaminase bilirubin test and glucose-6-phosphate dehydrogenase).

Exclusion criteria

  • Tobacco smoking.
  • Use of regular medications.
  • Had taken antibiotics for 4 weeks immediately preceding the study.
  • History of kidney stones, diabetes mellitus, bleeding disorders or family history of iron overload/hemochromatosis.

Treatment and study plan

raw broccoli

Other

To eat this plant material

Other names: Bimi, Kailan hybrid

Primary outcomes

  1. Metabolic fate of ITC of the kailan-hybrid broccoli after human comsuption

    Time frame: Up to 4 weeks

    Glucosinolates (sulphur-containing compounds) are hydrolyzed by the plant enzyme myrosinase to isothiocyanates (ITC), which have chemopreventive properties. Previous studies have shown that cooking process of vegetables may vary the human absorption of the correspondent nutritional and bioactive compounds.

Sponsors and collaborators

Lead sponsor

Universidad Politécnica de Cartagena

Other

Collaborators

  • Hospital Universitario Reina Sofia de Cordoba

Registry information

Official study title

Bioavailability of Bioactive Compounds in Broccoli

Important dates

Study start
2011
Primary completion
2011
Study completion
2011
First posted
Dec 6, 2012
Registry last updated
Dec 6, 2012

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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