Assessing the Expression and the Activity of Rac1 Protein in the Airway Smooth Muscle of Asthmatic Patient

Patients will be openly labeled in one of the following group

• 15 patients with severe asthma, without oral corticosteroids treatment.,
• 6 patients with mild to moderate asthma without treatment,
• 21 controlled samples from smooth muscle cells of non-asthmatic deceased donor Bronchial endoscopy with bronchial biopsies will be assessed in all asthmatic patients, Control samples will be obtained from tracheobronchial rings of cadaveric donor. Expression and activity of Rac1 in airway smooth muscle will be determined by immuno-staining on paraffinised biopsies.

Patients will undergo two study visits (D0: inclusion visit, D15: exploratory visit) and one phone call at D21.

Inclusion Visit (D0):

• Signature of the Informed consent,
• Medical History,
• Clinical examination,
• Pulmonary Function Test,
• Blood samples for fibroscopy safety,
• ACT (Asthma Control Test), ACQ (Asthma Control Questionnaire), AQLQ (Asthma Quality of Life Questionnaire), Morisky questionnaire

Exploratory visit (D15)

• Clinical examination,
• Bronchial endoscopy with biopsies Adverse event

Phone Call (D21)

• Adverse event

Recruitment will last 4 years.

Inclusion criteria

• Asthmatic Patient :

• Male or Female from 18 to 70 years old,
• Diagnosis of asthma confirmed by

• Existence of one or more following symptoms over 3 months at least (wheezing dyspnea, wheezing, chronic cough and tightness in the chest…)
• AND past pulmonary function test showing a reversible obstructive ventilatory syndrome after inhaled short-acting bronchodilators (improvement of FEV1 above 12% and at least 200 mL FEV1 gain compare to pre-bronchodilatator FEV1)
• AND a removal of the clinically suspected differential diagnoses of asthma (vocal cord dysfunction, Churg-Strauss syndrome etc…). The comorbidities should have been explored and treated or on treatment at enrollment.
• Subject agreed to participate to the study and the biological samples collection,
• Subject is affiliate to a social security system.
• Inclusion criteria for severe asthmatic patient :

Patient with one of the following criterion will be considered as severe asthmatic patient :

• Patient with a controlled asthma but using high dose of inhaled corticosteroids with another therapeutic classes,
• OR Patient with uncontrolled asthma despite treatment,
• OR Patient with worsening asthma despite treatment.

-->Inclusion criteria for non-severe asthmatic patients :

• Moderate Asthmatic patient without standard of care treatment since at least one week before the exploratory visit.

-->Controlled sample:

• Non asthmatic cadaveric adults

Exclusion criteria

• Asthmatic Patient :

• Underage,
• Pregnant or breast-feeding women,
• Adult on guardianship
• Active smoker (smoked or Inhaled),
• former smokers (smoked or Inhaled) with a smoking history of ≥10 pack years since less than 5 years.
• Patient with asthma exacerbation the 4 past weeks before the exploratory visit..
• Patient treated by long term oral corticosteroids or ongoing biological therapy or stopped within the 3 months before exploratory visit.
• Patient with severe acute or chronic organ disorder (cardiovascular, respiratory, hepatic, renal, malabsorption)
• Patient with history of unstable angina,
• Patient with platelet count abnormality, or primary or acquired thrombopathy, or an aPTT (activated Partial Thromboplastin Time) greater than or equal to 1,5 times normal or patient with a Quick Time > 26 seconds
• Patient under a systemic immunomodulatory or immunosuppressive treatment
• Patient with anticoagulants or anti-platelet aggregating drugs other than D-lysin acetylsalicylate and that could not be suspended before the bronchial fibroscopy.
• Patient with hypersensitivity to the treatment used during the bronchial fibroscopy: Hydroxyzine, Midazolam, Xylocaine.
• Patient with AME (Government Medical Assistance),
• Patient having physical and psychological disabilities to follow the protocol,
• Patient included in another interventional research protocol,
• Patient having risk factors of Creutzfeld-Jakob disease
• Controlled sample:

• Asthmatic patient