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Completed

NCT Number: NCT01912235

Arginine Synthesis in Healthy Adult Humans

Arginine synthesis in mammals is a complex process involving at least 8 enzymes and intracellular and interorgan transfer of substrates.

The investigators have shown in our neonatal studies that there is no conversion of glutamate to arginine but proline label appears in ornithine, citrulline and arginine. It is clear therefore that arginine is synthesized in human neonates from proline.

In adults the source of the carbon backbone is less clear.

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Key information

Conditions

Healthy

Age range

18 year–55 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

The Hospital for Sick Children

Toronto, Ontario, Canada

About this study

Through a series of test in healthy adults using tandem mass spectrometry with carbon labeled GLN and nitrogen labeled PRO to determine the dietary source for the carbon chain of ORN, CIT and ARG. Additionally, using Nitrogen labeled GLN, we will identify the source of the nitrogen atoms in these amino acids.

Using guanidino - 15N2 arginine, 1-13C Glutamine, 2-15N Glutamine and 15N Proline we will be able to:

  • determine the flux of arginine and proline in healthy adult volunteers
  • determine and compare the flux of carbon and nitrogen labeled glutamine
  • demonstrate whether glutamine or proline provides the carbon backbone for ornithine, citrulline and arginine in adults.
  • the source of the N atoms in ornithine, citrulline and arginine.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • 5 men of average weight 80 kg will be studied
  • healthy, with no know disease of liver, and/or kidney
  • weight stable

Exclusion criteria

  • weith loss or gain over the last three months
  • on medications known to affect amino acid metabolism
  • known liver, kidney disease condition

Treatment and study plan

2-15N glutamine

Other

Glutamine with N15 isotope tracer

15N2 arginine and 15N proline

Other

Arginine and Proline isotope tracers

1-13C glutamine

Other

Glutamine carbon isotope tracer

Primary outcomes

  1. Blood Isotope Levels

    Time frame: 8 hours

Sponsors and collaborators

Lead sponsor

The Hospital for Sick Children

Other

Registry information

Official study title

Arginine Synthesis in Healthy Adult Humans: Does Proline or Glutamate Provide the 5-Carbon Chain?

Important dates

Study start
2007
Primary completion
2008
Study completion
2008
First posted
Jul 31, 2013
Registry last updated
Jan 9, 2018

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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