Application of Topical Oxygen Therapy Around Customized Healing Collar

Interventions

Preoperative Measures Prior to the second-stage surgical procedure, all participants will undergo a standardized preoperative assessment to ensure their suitability for implant exposure and subsequent healing abutment placement.

The following components will be systematically conducted:

• Medical and Dental History Evaluation A comprehensive evaluation of each patient's medical and dental history will be performed to identify any systemic conditions or pharmacological agents that may compromise wound healing or elevate the risk of postoperative complications.
• Clinical and Radiographic Assessment A detailed clinical examination will be undertaken to evaluate the thickness of the soft tissue, the width of keratinized mucosa, and the condition of the mucosa overlying the implant site. Radiographic assessment using periapical radiographs or cone-beam computed tomography (CBCT), where indicated, will be conducted to confirm successful osseointegration of the dental implant and to exclude any evidence of peri-implant pathology.
• Oral Hygiene Evaluation and Instruction Oral hygiene status will be assessed using the Plaque Index. Patients will receive personalized oral hygiene instructions to ensure optimal plaque control before the surgical intervention. In cases where oral hygiene is deemed suboptimal, professional mechanical plaque removal (PMPR) will be provided, followed by re-evaluation prior to proceeding with the second-stage surgery.

Surgical Protocol

During the second-stage procedure, local anesthesia will be administered via infiltration using 2% lidocaine with 1:100,000 epinephrine. A mid-crestal or slightly palatal crestal incision will then be made to access the implant site.

Once osseointegration is clinically confirmed, the cover screw will be removed. Subsequently, a customized healing abutment(PEEK), appropriate to the implant diameter, will be placed and securely hand-tightened.

Application of Blue®M Gel:

Immediately following the placement of the healing abutment, Blue®M oral gel (Blue®M Europe BV, theNetherlands) will be applied circumferentially around the healing collar using a sterile microbrush. The gel will be gently massaged into the peri-implant mucosa and left in situ.

Postoperative instructions will include:

• Patients will be advised to refrain from rinsing or brushing the treated area for the first 12 hours.
• Blue®M gel application will be continued by the patient twice daily for 7 days postoperatively, in conjunction with standard oral hygiene measures.

Postoperative Instructions and Follow-Up Patients will receive standard postoperative instructions and will be prescribed 0.12% chlorhexidine mouthwash to be used twice daily for one week. Analgesics (e.g., ibuprofen 400 mg) will be administered as needed. If sutures are used, they will be removed after 7-10 days.

Patients will be clinically evaluated at baseline (day of second-stage surgery), day 7, and day 21 for the following:

Horizontal and linear Soft tissue change ,Patient-reported outcomes (pain, discomfort),Digital photographs .

Inclusion criteria

• Adults aged 21 years and above.

• Indicated for customized healing collar placement after second-stage surgery.
• Good general health.
• Able and willing to provide written informed consent.
• Committed to follow-up visits.

Exclusion criteria

• Individuals below 21 years old.
• Systemic diseases affecting healing.
• Smokers/tobacco users.
• Pregnant/lactating women.
• Medications affecting soft tissue healing.
• Chemotherapy/radiotherapy patients.