Application of 18F-CP6A PET Imaging in Synucleinopathies

Neurodegenerative diseases represent a significant global health challenge. With the increasing life expectancy, it is estimated that by 2050, the number of individuals suffering from dementia will reach 50 million. Among these diseases, Parkinson's disease (PD) is the second most common neurodegenerative disease, following Alzheimer's disease (AD). The typical pathological features of PD include the loss of dopaminergic neurons and the accumulation of α-synuclein (α-Syn), leading to the formation of Lewy bodies. Other diseases with similar pathological features of α-Syn accumulation include Dementia with Lewy Bodies (DLB) and Multiple System Atrophy (MSA). However, many neurodegenerative diseases, including those related to synucleinopathies, can present with similar parkinsonian syndromes, making differential diagnosis challenging.

α-Synuclein has emerged as a potential diagnostic and differential diagnostic biomarker for synucleinopathies. The deposition of α-Syn is a common characteristic of these diseases, and pathological examinations have shown significant heterogeneity in the deposition patterns across different synucleinopathies. In PD, α-Syn typically appears as intracellular inclusions within neurons, first appearing in the brainstem, then spreading to the midbrain/substantia nigra, and eventually to the medial temporal cortex and neocortex. In DLB, α-Syn distribution is more widespread in cortical regions compared to PD, while in MSA, α-Syn deposits are primarily found in oligodendrocytes, particularly in the white matter of the brainstem and cerebellum. Therefore, in vivo imaging of α-Syn could be an important tool for diagnosing and differentiating synucleinopathies.

Positron emission tomography (PET) has been widely used for diagnosing neurodegenerative diseases, using probes targeting amyloid β (Aβ) and tau proteins for AD. However, the deposition of α-Syn is usually less prominent than that of Aβ and tau. Additionally, dopamine transporter (DAT) and vesicular monoamine transporter type 2 (VMAT2) imaging are also used for diagnosing PD but are less effective for distinguishing atypical Parkinsonism or other conditions affecting the nigrostriatal pathway. These imaging techniques are also highly sensitive to the effects of dopaminergic medications, which can alter the imaging results. To address these challenges, researchers have developed highly selective α-Syn imaging agents, enabling early diagnosis and monitoring of disease progression in synucleinopathies.

This study aims to evaluate the safety, diagnostic potential, pharmacokinetics, and radiation dose of the α-Syn specific imaging agent, 18F-CP6A, in patients diagnosed with PD, MSA, and DLB, as well as healthy volunteers, using integrated PET/MRI imaging. The specific objectives are:

• Evaluate the safety of a single intravenous dose of 18F-CP6A in participants.
• Investigate the potential of 18F-CP6A in aiding the diagnosis of different synucleinopathies.
• Investigate the pharmacokinetics of 18F-CP6A in healthy volunteers.
• Investigate the radiation dose to the human body after a single intravenous injection of 18F-CP6A in healthy volunteers.

Inclusion criteria

Healthy Volunteers:

• Fully understand and voluntarily sign the informed consent form.
• Aged 18-65 years, any gender.
• Male weight ≥ 50.0 kg, female weight ≥ 45.0 kg; BMI within 19.0-26.0 kg/m2.
• Non-synucleinopathy, normal motor function, UPDRS score of 0.
• No history of other neurological or chronic diseases in the last 3 years.
• No abnormal vital signs or physical examination findings.
• No pregnancy or lactation, and willing to use effective contraception during the trial period and for 6 months post-study.
• No participation in other clinical trials within the past month.
• Able to communicate effectively with the study team and understand and comply with the study requirements.

Synucleinopathy Patients:

• Ability to sign the informed consent form or by a legal representative.
• Aged 50-80 years, any gender.
• Clinically diagnosed or suspected PD, MSA, or DLB based on diagnostic criteria [including China's Parkinson's Disease Diagnosis (2016), MSA Consensus (2022), and DLB Guidelines (2021)].
• No other neurological diseases, severe chronic diseases, or malignancies.
• No family history of neurodegenerative diseases or movement disorders.
• Female participants of childbearing potential must use contraception throughout the study period.

Exclusion criteria

Healthy Volunteers:

• Any severe or unstable medical condition, acute diseases prior to the study.
• Pregnant or breastfeeding women.
• Any surgery within the last 6 months that could affect drug absorption, distribution, metabolism, or excretion.
• Use of any medication (prescription or over-the-counter) within 2 weeks prior to the study.
• Inability to undergo PET/MRI or PET/CT imaging (e.g., claustrophobia).
• Participation in studies involving radioactive substances within the last 12 months.
• Any other factor that may interfere with study results.

Synucleinopathy Patients:

• Inability or unwillingness to sign the informed consent form.
• Pregnant or breastfeeding participants.
• History of other neurological diseases or severe chronic illnesses.
• Known allergy to α-Syn imaging agents or synthetic excipients.
• Inability to complete PET/MR or PET/CT imaging.
• Researcher assesses poor compliance or other conditions that would render participation unsuitable.
• Major surgery within the last month.
• Participation in other clinical trials within the last 3 months.