Antibacterial Activity of Urea Against… | Clinical Trial

Key information

Conditions

Urea in Blood; High Female Urogenital Diseases Female Urogenital Diseases and Pregnancy Complications Kidney Diseases Male Urogenital Diseases Uremia Urogenital Diseases Urologic Diseases

Age range

5 month–65 year

Sex eligibility

All sexes

Study type

Observational

Primary location

Hassan Lotfy Fahmy

Asyut, Egypt

About this study

In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.

Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.

Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

ocular infection

Exclusion criteria

Local antibiotics treatment

Treatment and study plan

Antibacterial Activity

Biological

Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates

Primary outcomes

Antibacterial effect of Urea Solution against Ocular Bacterial

Time frame: 6 month

Samples were both inoculated on Muller Agar (Sigma-Aldrich) plates and directly examined under the microscope using Gram stained smears. Inoculated plates were incubated at 37oC for 48 hrs.

Gram negative isolates were sub-cultured on MacConkey's, Eosin Methelyene Blue (EMB) and Cystine Lactose Electrolyte Deficient (CLED) agar plates (Sigma-Aldrich). Gram positive isolates were sub-cultured on Mannitol Salt agar plates (Sigma-Aldrich).

Sponsors and collaborators

Lead sponsor

Assiut University

Other

Registry information

Official study title

In Vitro Evaluation of Antibacterial Activity of Urea Solution Against Ocular Bacterial Isolates

Important dates

Study start: 2017
Primary completion: 2018
Study completion: 2018
First posted: Jul 16, 2019
Registry last updated: Jul 16, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

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This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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Completed