Advanced Sperm Selection in ICSI: A Comparative Study of PICSI and ZyMot

Inclusion criteria

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• Age for women is between 20-39 years old.
• Body mass index (BMI) between 19 kg/m and 35 kg/m
• Normal Prolactin level.
• No other endocrinological abnormality including thyroid disorder or diabetes.
• With antimullerian hormone (AMH) at least 1.5 ng/ml, ), with normal progesterone level.
• Primary or secondary infertility at least of 2 years duration.
• No evidence of endometriosis.
• No other medical or surgical disease.
• The day of embryo freezing is the fifth day (day 5), and all patients who undergo the process of transferring frozen embryos.
• The endometrium thickness is between 8-14 mm on day of transfer.
• Inclusion criteria of male partner were; presence of ejaculate motile spermatozoa with total sperm number 1 million/ml at least and sperm motility 5% or more and all patient with high DNA fragmentation index above 35%.

Exclusion criteria

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• Women were older than 40 years of age or younger than 18 years old.
• Women laparoscopically diagnosed to have endometriosis within the last 1 year.
• Male infertility factor with immotile sperm.