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Completed

NCT Number: NCT02420886

Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation

Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.

Completed

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Key information

Conditions

Infertility Genital Diseases Urogenital Diseases

Age range

18 year–40 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

Banon IVF Center, Asyut, Egypt

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About this study

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • all patients

Exclusion criteria

  • over 40 years

Treatment and study plan

Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media

Other

Primary outcomes

  1. ongoing pregnancy rate ( % )

    Time frame: 6 months

Secondary outcomes

  1. clinical pregnancy rate ( % )

    Time frame: 6 months

  2. implantation rate

    Time frame: 6 months

  3. fertilization rate

    Time frame: 6 days of culture

  4. top-quality embryo rate

    Time frame: 6 days of culture

  5. rates for blastocyst formation and quality

    Time frame: 6 days of culture

  6. embryo utilization rate

    Time frame: 6 days of culture

Sponsors and collaborators

Lead sponsor

Ibn Sina Hospital

Other Gov

Collaborators

  • Banon IVF Center Assiut, Egypt

Registry information

Official study title

The Effects of GM-CSF, HB-EGF and LIF Cytokines Supplementation Need Verification for Human Embryo Development in Vitro

Acronym: GHLCSHED

Important dates

Study start
2015
Primary completion
2016
Study completion
2016
First posted
Apr 20, 2015
Registry last updated
Oct 2, 2018

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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