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Completed

NCT Number: NCT04648384

Acute Whey Protein And Casein Supplementation: Effect On Protein Metabolism After Resistance Exercise

The proteins of human breast milk are the most important endocrine signaling system that promotes neonatal growth by increasing the release of insulin, insulin-like growth factor-1 (IGF-1), and the leucine-mediated mammalian target of rapamycin complex 1- (mTORC1) signaling of pancreatic β-cells. Remarkably, the branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are involved in the growth-promoting effects of milk, protein biosynthesis, and metabolism because they physiologically stimulate insulin secretion The present study sought to investigate the effects of the combination of whey protein and casein in the ratio of 80:20 ("whey protein: casein" or "casein: whey protein") as breast milk proportion on the peak and the period of the permanence of branched-chain amino acids in the blood circulation, final metabolites of protein metabolism and delayed onset muscle soreness (DOMS) after a single bout of fasting or a resistance exercise session.

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Key information

Conditions

Sports Nutrition Protein Supplementation

Age range

20 year–40 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

University of São Paulo, School of Physical Education and Sports of Ribeirão Preto.

Ribeirão Preto, São Paulo, 14049-900, Brazil

About this study

The present study investigated the effects of the association of WP and casein in the ratio of 80:20, a similar ratio of human breast milk, on blood branched-chain amino acids (BCAAs) profile by HPLC technique, markers of protein metabolism (creatinine, urea, and urine nitrogen) and delayed onset muscle soreness (DOMS) after a single bout of resistance exercise. A double-blind, crossover and acute study was conducted with ten men (age 29 ± 8 y; BMI: 25.4 ± 2.9 Kg/m2; 77 ± 12 kg; 1.74 ± 0.09 m) that were randomly assigned to five supplementation treatments: WP - whey protein; CAS - casein; WP/CAS - 80% WP/20% CAS; CAS/WP - 80% CAS/ 20% WP; PLA - placebo. Participants were submitted to the following evaluations: performance sessions, blood collection for each session for the BCAAs profile determination, two food records, 3-days assessment of DOMS (24h, 48h, 72h) after each treatment.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy
  • practice resistance training at least 3x a week

Exclusion criteria

  • Smokers
  • Muscle injures
  • cardiovascular diseases
  • Diabetes mellitus
  • Lactose intolerance

Treatment and study plan

Blend Protein Supplementation

Dietary Supplement

Protein supplementation using 20 grams of whey protein or casein mix. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Other names: Resistance Training

Placebo supplementation

Dietary Supplement

Placebo supplementation using 20 grams of Maltodextrin. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Other names: Resistance Training

Resistance Exercise

Other

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Primary outcomes

  1. Change from baseline in plasma amino acids concentration

    Time frame: 5 weeks

    Blood sample collected for analysis of BCAA concentration after blend protein or placebo supplementation expressed in (µmol/L)

  2. Change from baseline in 1RM test

    Time frame: 5 weeks

    Change of training load using RPE, time and load expressed in (a.u)

  3. Change in protein metabolism

    Time frame: 5 weeks

    Change in nitrogen excretion, and nitrogen balance after protein supplementation expressed in (g/24 hours)

  4. Changes from baseline in macronutrient intake

    Time frame: 5 weeks

    Change of macronutrient intake were calculated pre and post treatment expressed in (g/24 hours)

  5. Change in delayed onset muscle soreness

    Time frame: 5 weeks

    Change from baseline the delayed onset muscle soreness pre, post, 24 hours, 48 hours and 72 hours after treatment expressed in (a.u)

Sponsors and collaborators

Lead sponsor

University of Sao Paulo

Other

Collaborators

  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
  • Fundação de Amparo à Pesquisa do Estado de São Paulo
  • Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (COLCIENCIAS)

Registry information

Official study title

Association of Whey Protein and Casein in the Same Proportions of Human Breast Milk Promoted Similar Branched-chain Amino Acids Profile and Reduced Delayed Muscle Soreness in Physically Active Man

Important dates

Study start
2016
Primary completion
2019
Study completion
2019
First posted
Dec 1, 2020
Registry last updated
Dec 2, 2020

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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