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NCT Number: NCT02590913

Acute Metabolic and Endocrine Responses of Glucose and Fructose in Healthy Young Subjects

The purpose of this study is to investigate the metabolic and endocrine responses during 3 hour after taking 75g fructose or glucose in young Chinese.

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Key information

Conditions

Healthy

Age range

19 year–39 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Zhejiang university

Hangzhou, Zhejiang, 310000, China

About this study

The study was a double-blinded, randomized, crossover trial. Twenty healthy volunteers were randomized to two groups: group A (300ml of 22.5% glucose) and group B (300ml of 22.5% fructose).Volunteer was randomized into either group A or B, after an 8-h overnight fasting, took 300ml of solution at 0830 within 5 minutes. After one-week washout period, volunteer was crossed over for either group B or A. each intervention, venous blood was collected at 0h, 0.5h, 1h, 2h, and 3h and blood pressure was tested at 0h, 1h, 2h, and 3h.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Inclusion criteria were aged 19-39 years, with BMI between 18-24 kg/m 2.

Exclusion criteria

  • Subjects pregnant or having a history of hepatic or renal disease, fructose intolerance, gout, hypoglycemia, or diabetes mellitus were excluded.
  • Subjects taking any drugs, botanicals, or nutraceutical known to influence blood pressure also were rejected either.

Treatment and study plan

Fructose

Dietary Supplement

75g fructose is dissolved in 300 ml water.After overnight fasting, 300ml of solution at 0830 within 5 minutes is taken by volunteers

Glucose

Dietary Supplement

75g glucose is dissolved in 300 ml water.After overnight fasting, 300ml of solution at 0830 within 5 minutes is taken by volunteers

Primary outcomes

  1. serum uric acid

    Time frame: 3 hours

    Serum uric acid concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by DiaSys Diagnostic Systems (Shanghai) Co., Ltd

Secondary outcomes

  1. blood pressure

    Time frame: 3 hours

    Blood pressure was measured at Zhejiang University Hospital by the research workers who were blind to the groups within 3 hours blood collection.

Other outcomes

  1. serum glucose

    Time frame: 3 hours

    Serum glucose level was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd

  2. nitric oxide

    Time frame: 3 hours

    Serum nitric oxide concentration was measured using NO assay kit (Nanjing Jiancheng Technology Co., Lte., Nanjing, China) by the research workers who were blind to the groups.

  3. aldose reductase

    Time frame: 3 hours

    Enzymatic activity of aldose reductase in serum was measured using ELISA kit (Shanghai Changjin Biology Co., Lte., Shanghai, China ) by the research workers who were blind to the groups.

  4. phosphofructokinase

    Time frame: 3 hours

    Enzymatic activity of phosphofructokinase in serum was measured using ELISA kit (Shanghai Changjin Biology Co., Lte., Shanghai, China ) by the research workers who were blind to the groups .

  5. insulin

    Time frame: 3 hours

    Serum insulin concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd

  6. insulin-c

    Time frame: 3 hours

    Serum insulin-c concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd

  7. triglyceride

    Time frame: 3 hours

    Triglyceride concentration in serum was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd

  8. total cholesterol

    Time frame: 3 hours

    Total cholesterol concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd

  9. high-density cholesterol

    Time frame: 3 hours

    High-density cholesterol was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd.

  10. low-density cholesterol

    Time frame: 3 hours

    Low-density cholesterol concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd.

  11. lactate dehydrogenase

    Time frame: 3 hours

    Enzymatic activity of lactate dehydrogenase was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd.

Sponsors and collaborators

Lead sponsor

Zhejiang University

Other

Registry information

Official study title

Acute Metabolic and Endocrine Responses of Glucose and Fructose in Healthy Young Subjects: A Double-blinded, Randomized, Crossover Trial

Important dates

Study start
2015
Primary completion
2015
Study completion
2015
First posted
Oct 29, 2015
Registry last updated
Oct 29, 2015

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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