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Completed

NCT Number: NCT07589712

Acute Effects of Proprioceptive Neuromuscular Facilitation on Hamstring-to-Quadriceps Strength Ratio

This study aimed to compare the acute effects of proprioceptive neuromuscular facilitation (PNF) and static stretching on hamstring-to-quadriceps (H/Q) strength ratios in healthy young men. A randomized crossover design was used in which participants completed both stretching protocols on separate days. Isokinetic muscle strength and H/Q ratios were assessed following each intervention. The findings provide insight into the immediate effects of different stretching modalities on muscle balance and performance.

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Key information

Age range

18 year–25 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Gumushane Univetsity

Gümüşhane, Trabzon, Ordu, Giresun, Rize, Artvin, Gümüşhane, 29600, Turkey (Türkiye)

About this study

The present study investigated the acute effects of two commonly used stretching modalities-proprioceptive neuromuscular facilitation (PNF) and static stretching-on isokinetic muscle strength and hamstring-to-quadriceps (H/Q) ratios in physically active healthy young males. The study employed a randomized crossover design with repeated measures, allowing each participant to complete both experimental conditions under standardized laboratory settings.

Participants attended three sessions: a familiarization session followed by two experimental sessions separated by at least 48 hours. Each experimental session included a standardized warm-up, followed by either PNF or static stretching intervention, and subsequent isokinetic strength testing. Peak torque values for quadriceps (concentric) and hamstrings (concentric and eccentric) were measured using an isokinetic dynamometer at 60°/s. Traditional and functional H/Q ratios were calculated.

The primary aim was to determine whether these stretching modalities produce acute alterations in muscle strength and H/Q ratios. The study also examined whether the type of ratio (traditional vs. functional) significantly influences outcomes. Findings contribute to understanding the neuromuscular implications of stretching in warm-up protocols.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Age between 18 - 25 years, healthy male individuals
  • Physically active (≥3 days/week, ≥30 min/day)

Exclusion criteria

  • Musculoskeletal injury in the last 6 months
  • Any neurological or orthopedic disorder

Treatment and study plan

PNF Stretching

Other

Participants performed four repetitions of PNF stretching consisting of a 6-second isometric hamstring contraction at approximately 60% of maximal voluntary contraction followed by a 30-second passive stretch. Rest intervals were standardized.

Static Stretching

Other

Participants performed four repetitions of static hamstring stretching, each lasting 30 seconds, with 30-second rest intervals between repetitions.

Primary outcomes

  1. Hamstring-to-Quadriceps (H/Q) Ratio

    Time frame: Immediately after intervention

    Both traditional (Hcon/Qcon) and functional (Hecc/Qcon) ratios were calculated using isokinetic peak torque values.

Secondary outcomes

  1. Quadriceps Concentric Peak Torque

    Time frame: Immediately post-intervention

    Quadriceps Concentric Peak Torque

  2. Hamstring Concentric Peak Torque

    Time frame: Immediately post-intervention

    Hamstring Concentric Peak Torque

  3. Hamstring Eccentric Peak Torque

    Time frame: Immediately post-intervention

    Hamstring Eccentric Peak Torque

Sponsors and collaborators

Lead sponsor

Gümüşhane Universıty

Other

Registry information

Official study title

Acute Effects of Proprioceptive Neuromuscular Facilitation and Static Stretching on Hamstring-to-Quadriceps Strength Ratio in Healthy Young Men

Acronym: Acute Effects

Important dates

Study start
2025
Primary completion
2025
Study completion
2025
First posted
May 15, 2026
Registry last updated
May 15, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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