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NCT Number: NCT06868862

Acute Effects of Isoenergetic HIIE Vs. MICE on Key Parameters of Fat Mass Loss in Young Females with Overweight or Obesity

Overweight and obesity, particularly increased total and/or abdominal fat mass (FM), are strongly linked to the development of cardiovascular diseases. Managing obesity effectively requires lifestyle and dietary modifications, including increased physical activity. Among the various exercise strategies, Moderate-Intensity Continuous Training (MICT) and High-Intensity Interval Training (HIIT) are widely recommended for reducing FM. Compared to MICT, HIIT is a time-efficient approach, often perceived as more enjoyable, and has been shown to promote greater total and abdominal fat loss in a shorter period. However, the precise mechanisms underlying this enhanced fat loss remain unclear.

This study aims to compare the acute effects of two isoenergetic exercise sessions - MICE (Moderate-Intensity Continuous Exercise) and HIIE (High-Intensity Interval Exercise) - on key physiological responses involved in fat mass loss. Specifically, we will assess plasma catecholamine levels during exercise and recovery, as well as oxygen consumption, substrate oxidation, and appetite over the 2-hour post-exercise period in young females with overweight or obesity. By elucidating the role of catecholaminergic stimulation as a potential regulator of fat mass loss, this research could provide valuable insights for optimizing exercise strategies in weight management and metabolic health.

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Key information

Age range

20 year–45 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

Laboratório de Fisiologia e Metabolismo Aplicados à Educação Física

São Paulo, 475, Brazil

Location status: Recruiting

Location contact

JULIANA MONIQUE LINO APARECIDO, PhD

CONTACT

[email protected]

(+55) 1196997-5162

Nathalie Boisseau, Professor

CONTACT

[email protected]

(+33) 0684331693

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • women with overwieght or obesity (BMI: 25-35 kg/m²)

Exclusion criteria

  • contraceptive use
  • menopause
  • irregular menstrual cycle
  • medical contraindications to intense physical activity,
  • painful joints,
  • taking ß-blocker

Treatment and study plan

Exercise

Other

Two Exercise Modalities: HIIE and MICE

Primary outcomes

  1. Oxygen consumption during exercise and the recovery period

    Time frame: Measurement at rest for 20 minutes, then during warm-up for 10 minutes, then during exercise for 20 minutes and finally during 2 hours after the end of the exercise.

    Determination of oxygen consumption during exercise and 2 hours after the exercise session (HIIE and MICE). Determination from Metamax (3D Cortex).

Secondary outcomes

  1. Lipid before and after the exercise.

    Time frame: Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.

    Determination of lipid oxidation before and after the exercise. Lipid oxidation is measured from oxygen and carbon dioxide consumption (Metamax 3D Cortex).

  2. Carbohydrate before and after the exercise.

    Time frame: Measurement at rest for 20 minutes and then during 2 hours after the end of the exercise.

    Determination of carbohydrate oxidation before and after the exercise. Carbohydrate oxidation is measured from oxygen and carbon dioxide consumption (Metamax 3D Cortex).

  3. Catecholamines

    Time frame: 5 collections: before exercise started, at the end of exercise (exercise duration is 20-35 minutes), at 30 minutes, 60 minutes and 120 minutes after the end of exercise

    Determination of cathecolamines concentration in blood during exercise and 2 hours after the end of the exercise.

  4. Energy intakes 24 hours after the exercise

    Time frame: 24 hours post exercise

    Evaluation of energy intakes after the exercise session (HIIE vs MICE). Exercise session finish at 11:30 a.m. After that, at home, participants have to complete by themselves a food-record on which they will describe all their meal during 24 hours after the end of the exercise session. It includes their lunch, dinner and their breakfast the next morning.

Sponsors and collaborators

Lead sponsor

Laboratoire des Adaptations Métaboliques à l'Exercice en conditions Physiologiques et Pathologiques

Other

Collaborators

  • LAPEF laboratory, Sao paulo

Registry information

Official study title

Acute Physiological Responses to Isoenergetic High-Intensity Interval Vs. Moderate-Intensity Continuous Exercise in Overweight or Obese Young Females: Focus on Catecholamines, Oxygen Consumption, Lipid Utilization, and Appetite Regulation

Acronym: COMEFIT

Important dates

Study start
2025
Primary completion
2025
Study completion
2025
First posted
Mar 11, 2025
Registry last updated
Mar 11, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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