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Completed

NCT Number: NCT07051291

Acute Effects of Instant Coffee in Capsule vs Beverage Form on Plasma Metabolites

This study is designed to evaluate the acute effect of consuming a cup of instant coffee on circulating metabolites and to evaluate whether ingestion of instant coffee in the form of a capsule has a similar effect on circulating metabolites as compared to the conventionally prepared beverage.

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Key information

Conditions

Coffee

Age range

21 year–64 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Saw Swee Hock School of Public Health, National University of Singapore

Singapore, 117549

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Healthy individuals aged 21-64 years old of Chinese ethnicity and are physically-abled, can participate in the research, and are able to understand English

Exclusion criteria

  • Pregnant or breastfeeding
  • Certain medications including Zanaflex, Sirasalud, Ketanest S, Spravato and Ephedrine Sulfate
  • Any pre-existing medical conditions, depression, anxiety, caffeine/lactose intolerant, fear of needles and/or blood disorders, allergy to coffee or bovine capsules
  • Existing dietary restrictions which makes them unable to consume non-halal certified bovine gelatin (the capsule encasing the coffee powder)
  • Recent history of major surgical operations in the past year
  • Past or current user of oral/systemic hormone treatment or therapies
  • Taken antibiotics in the last 3 months
  • Taking nutritional supplements (that contains coffee/tea-related compounds naturally found in other plants)
  • Not willing to travel to the University for the study
  • Unable to commit to completing both study visits

Treatment and study plan

Instant Coffee Capsule

Dietary Supplement

Participants received 1.7 grams of instant coffee in the form of three capsules. 200 mL of plain water was provided to participants to swallow the three capsules.

Instant Coffee Beverage

Dietary Supplement

Dissolved 1.7 grams of instant coffee in 200 mL of hot water to make a coffee beverage.

Primary outcomes

  1. Caffeine and polyphenol metabolites

    Time frame: The period between the two study visits ranged between 7 to 28 days.

    Each participant underwent two interventions, and blood was collected pre- and post- each intervention. Blood was allowed to clot at room temperature. After 30-40 min, serum was separated from entire blood by centrifugation (3000g, 15 min, 4°C) and aliquoted into tubes. All samples were processed on the same day of collection. A total of 7 biomarker compounds (trigonelline, paraxanthine, theophylline, theobromine, caffeine, cyclo(Leu-Pro), and cyclo(Pro-Val)) were quantified using a targeted method developed in-house. The quantification method was optimized by LC retention elution, parent-daughter quantifier mass transitions, and varying MS cone and collision energies (in V) to obtain clear peak separation and height. Multiple Reaction Monitoring (MRM) in positive electrospray ionization mode was performed on a Waters Xevo TQ-S Triple Quadrupole Mass Spectrometer system.

Sponsors and collaborators

Lead sponsor

National University of Singapore

Other

Registry information

Official study title

The Acute Effect of Instant Coffee in Different Forms on Plasma Metabolites

Important dates

Study start
2020
Primary completion
2020
Study completion
2022
First posted
Jul 4, 2025
Registry last updated
Jul 4, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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