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NCT Number: NCT07260630

Acute Effects of Different Soft Tissue Techniques on Hamstring Tightness

Flexibility is one of the key components of health-related physical fitness and is influenced by various factors such as age, gender, joint structure, and muscle anatomy. Hamstring tightness can contribute to multiple lower extremity injuries. While traditional methods like stretching and myofascial release are commonly used to improve flexibility, instrument-assisted soft tissue mobilization (IASTM) has recently gained popularity. However, no studies in the current literature have compared the acute effects of these three methods in individuals with hamstring tightness. This study aims to compare the acute effects of stretching, myofascial release, and IASTM in individuals diagnosed with hamstring tightness (defined as <65° of hip flexion in the straight leg raise test without neurological findings).

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Key information

Conditions

Age range

18 year–30 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Gazi University

Ankara, Turkey (Türkiye)

About this study

Hamstring tightness is a common musculoskeletal problem that can contribute to lower extremity injuries and functional limitations. Flexibility, one of the fundamental components of physical fitness, can be influenced by multiple factors including age, sex, joint structure, and muscle properties. Among various therapeutic approaches, static stretching and myofascial release are conventionally used to improve hamstring flexibility. More recently, instrument-assisted soft tissue mobilization (IASTM) has gained popularity for its potential to increase range of motion and reduce muscle tension more rapidly than traditional methods.

The study aims to identify which intervention is most effective in increasing hamstring flexibility acutely and will provide evidence to guide clinical decision-making in sports rehabilitation and injury prevention. Additionally, this research intends to fill a gap in the literature, as no prior study has directly compared these three methods in individuals with hamstring tightness.

This study is designed as a prospective, randomized, controlled, double-blind trial to compare the acute and short-term effects of three different manual therapy interventions such as static stretching, self-myofascial release using a vibrating foam roller, and IASTM on individuals with clinically diagnosed hamstring tightness. A fourth group will serve as the control group and will receive no intervention.

A total of 36 healthy participants, aged 18 to 30 years, with hamstring tightness confirmed via the Straight Leg Raise (SLR) test (hip flexion <65° without neurological findings), will be randomly assigned into one of four groups. Each group will consist of 9 participants. Interventions will be applied for approximately 5 minutes. Assessments will be conducted at four time points: baseline (pre-intervention), immediately after intervention, 12 hours post-intervention, and 24 hours post-intervention.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Age between 18 and 30 years
  • Presence of hamstring tightness (Straight Leg Raise angle < 65°)
  • No diagnosed chronic diseases
  • Voluntary participation and signed informed consent

Exclusion criteria

  • History of lower extremity trauma or surgery
  • Symptoms radiating to one or both legs due to hamstring, spinal, or sciatic nerve injury
  • Ankle instability
  • Neurological findings during the straight leg raise test
  • Presence of hyperalgesia, hematoma, varicose veins, or skin infection in the lower limb

Treatment and study plan

Stretching group

Other

Manual static stretching applied to the hamstring muscle in five 30-second sets, with 30-second rest intervals. Total application time is 5 minutes.

Self-myofascial release

Other

Vibrating foam roller (38 Hz) used for self-myofascial release applied to hamstring muscles. Three sets of 30 seconds, with 30 seconds rest between sets.

Instrument-assisted Soft Tissue Mobilization

Other

Application of IASTM using a concave-edged tool over the posterior thigh for 5 minutes to increase tissue mobility and flexibility.

Primary outcomes

  1. Change in Hamstring Flexibility

    Time frame: From enrollment to the end of treatment at 1 weeks

    Hamstring flexibility will be evaluated using the passive and active Straight Leg Raise (SLR) test. An angle <65° indicates tightness. Change in hip flexion range will be measured at each time point.

  2. Change in Knee Extension Angle

    Time frame: From enrollment to the end of treatment at 1 weeks

    With the hip maintained at 90° flexion in a supine position, the passive and active knee extension angle will be measured using a goniometer. This test assesses the passive extensibility of the hamstring muscles.

Secondary outcomes

  1. Change in Hamstring Muscle Strength

    Time frame: From enrollment to the end of treatment at 1 weeks

    Maximal isometric hamstring muscle strength will be measured using a handheld dynamometer.

  2. Change in Quadriceps Muscle Strength

    Time frame: From enrollment to the end of treatment at 1 weeks

    Maximal isometric quadriceps strength will be assessed to evaluate potential reciprocal inhibition effects.

  3. Functional Performance - Vertical Jump Test

    Time frame: From enrollment to the end of treatment at 1 weeks

    Vertical jump performance will be measured to assess functional changes in lower extremity power.

  4. Functional Performance - Single-Leg Hop Test

    Time frame: From enrollment to the end of treatment at 1 weeks

    Single-leg horizontal hop distance will be recorded to evaluate functional lower limb performance.

  5. Subjective Perception - Global Rating of Change Scale

    Time frame: From enrollment to the end of treatment at 1 weeks

    Participants will rate their perception of change in hamstring flexibility using a 15-point Global Rating of Change Scale.

Sponsors and collaborators

Lead sponsor

Gazi University

Other

Collaborators

  • The Scientific and Technological Research Council of Turkey

Registry information

Official study title

Comparison of the Acute Effects of Stretching, Myofascial Release, and Instrument-Assisted Soft Tissue Mobilization in Individuals With Hamstring Tightness: Randomized Double-Blind Study

Important dates

Study start
2025
Primary completion
2026
Study completion
2026
First posted
Dec 3, 2025
Registry last updated
Dec 3, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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