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OpenTrials
Completed

NCT Number: NCT06675305

Acute Effects of Beetroot Juice Ingestion on Cognitive Function and Neuromuscular Performance in Elite Female Taekwondo Athletes

Beetroot juice is a good source of nitrate (NO3-), which is a precursor of nitric oxide (NO) through the NO3- to nitrite (NO2-) to NO pathway (Lundberg et al., 2008). Consequently, it is thought that a minimum threshold may exist for dietary NO3- consumption of at least 5 mmol to enhance athletic performance (Senefeld et al., 2020). In turn, systematic reviews have documented that the use of beetroot juice supplementation enhances muscle strength and power-related attributes (Gonzalez et al., 2023), as well as performance in repeated high-intensity activity bouts (Alsharif et al., 2023; Dominguez et al., 2018). However, beetroot juice supplementation has been shown to have equivocal effects on neuromuscular performance specifically among individual sport athletes (e.g. taekwondo). Thus, the aim of this study was to determine the effects of acute beetroot juice ingestion (140 mL, 12.8 mmol NO3-) on neuromuscular performance in elite female taekwondo athletes.

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Key information

Age range

16 year–40 year

Sex eligibility

Female

Study type

Interventional

Phase

Not applicable

Primary location

Universida Pontificia Comillas

Madrid, Spain

About this study

Beetroot juice is a good source of nitrate (NO3-), which is a precursor of nitric oxide (NO) through the NO3- to nitrite (NO2-) to NO pathway (Lundberg et al., 2008). This pathway is thought to promote increased sarcoplasmic reticulum calcium release and re-uptake with ensuing enhancements in force output within type II muscle fibers (Hernandez et al., 2012), as well as increased neurotransmitter release (Esen et al., 2022) and attenuated muscle potassium efflux (Wylie et al., 2013) that could benefit neuromuscular performance. Consequently, it is thought that a minimum threshold may exist for dietary NO3- consumption of at least 5 mmol to enhance athletic performance (Senefeld et al., 2020). In turn, systematic reviews have documented that the use of beetroot juice supplementation enhances muscle strength and power-related attributes (Gonzalez et al., 2023), as well as performance in repeated high-intensity activity bouts (Alsharif et al., 2023; Dominguez et al., 2018). However, beetroot juice supplementation has been shown to have equivocal effects on neuromuscular performance specifically among individual sport athletes (e.g. taekwondo). Thus, the aim of this study was to determine the effects of acute beetroot juice ingestion (140 mL, 12.8 mmol NO3-) on neuromuscular performance in elite female taekwondo athletes.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Age between 16 to 40 years
  • Competed in international taekwondo competitions representing her national teams.
  • More than6 years of training experience (at least 3 sessions per week).
  • Female taekwondo athletes.

Exclusion criteria

  • Male taekwondo athletes.

Treatment and study plan

Beetroot juice (James White, UK)

Dietary Supplement

One serving 140 mL of BJ (12.8 mmol of NO3-; Beet-It-Pro Elite Shot, James White Drinks Ltd., Ipswich, UK) after an overnight fast and 2.5 h before initiating the testing session.

Placebo acute supplementation (James White, UK)

Dietary Supplement

One serving 140 mL of BJ (0.04 mmol of NO3-; Beet-It-Pro Elite Shot, James White Drinks Ltd., Ipswich, UK) after an overnight fast and 2.5 h before initiating the testing session.

Other names: Beetroot juice placebo

Primary outcomes

  1. Simulated taekwondo Combat (actions/time)

    Time frame: 1-week

    Simulated taekwondo combat were performed according to the guidelines of the World Taekwondo (WT), and participants were divided based in the Olympic weight categories (WT, 2016). All the combats were executed in an official octagonal and homologated competition area while an international referee (who doesn´t know the experimental treatments) arbitrated. Today the rules of WTF led to use electronic protectors as protecting of athletes and identify electronically the punctuations (Del Vecchio, Franchini, Del Vecchio, & Pieter, 2011). Variables analyzed were attack time (AT), step time (ST), pause time (PT), period without attack (PWA = ST+PT), numbers of attacks (AN), the ratio for attack time and step time (R1 = AT/ST), and ratio for action time and step time (R2 = (AT+ST) /PT).

Secondary outcomes

  1. Changes in maximal jump height (cm)

    Time frame: 1-week

    Maximal jump height using a contact platform

  2. Reaction time (ms)

    Time frame: 1-week

    Reaction times in a specific reaction test with BlazePod ((Play Coyotta Ltd., Tel Aviv, Israel)

  3. Blood lactate concentrations (mmol·L-1)

    Time frame: 1-week

    Previous to the warm-up, just to the end of the simulated combat, and 3 minutes after, a researcher extracted a blood sample of the pad of the index finger of the left hand of the participants.

  4. Rating of percevied exertion (RPE) (measured using scale 6-20 points)

    Time frame: 1-week

    Immediately after each one of the 3 assaults, participants were asked for providing her RPE using a Borg scale from 6 to 20 points

Sponsors and collaborators

Lead sponsor

Universidad Pontificia Comillas

Other

Collaborators

  • Universidad Complutense de Madrid
  • University of Seville

Registry information

Acronym: BEET_TAEKWONDO

Important dates

Study start
2022
Primary completion
2022
Study completion
2022
First posted
Nov 5, 2024
Registry last updated
Nov 5, 2024

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.