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NCT Number: NCT07800156

The Effect of Aerobic Exercise on the Irisin/Klotho/Sirtuin-1/BDNF Axis

The purpose of this study is to investigate the effects of an acute aerobic exercise on irisin, klotho, sirtuin-1, and BDNF levels in the healthy adults. The findings will help understand the physiological impacts of exercise on these biomarkers.

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Key information

Conditions

Healthy Adult Athlete

Age range

18 year–45 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Gaziantep University Faculty of Sports Sciences

Gaziantep, Sehitkamil, 85142, Turkey (Türkiye)

Location contact

CEMRE

CONTACT

[email protected]

05452645486

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Being between the ages of 18 and 45
  • Being in good health and having no diagnosis of any chronic disease
  • Not having taken regular medication within the last 4 weeks
  • Voluntary participation and providing written informed consent

Exclusion criteria

  • Individuals with a history of cardiovascular, endocrine, metabolic, or renal disease
  • Individuals with a history of active infection, musculoskeletal injury, or severe disability
  • Individuals with any medical condition that might prevent participation in exercise
  • Individuals unable to regularly participate in the exercise protocol throughout the study

Treatment and study plan

Aerobic Exercise

Behavioral

Acute aerobic exercise until exhaustion

Primary outcomes

  1. Salivary Irisin Level

    Time frame: Baseline- immediately after exercise and 1 hours after exercise

    Irisin concentration will be determined from a saliva sample using the ELISA technique.

  2. Salivary Klotho Level

    Time frame: Baseline- immediately after exercise and 1 hours after exercise

    Klotho concentration will be determined from a saliva sample using the ELISA technique.

  3. Salivary Sirtuin Level

    Time frame: Baseline- immediately after exercise and 1 hours after exercise

    Sirtuin concentration will be determined from a saliva sample using the ELISA technique.

  4. Salivary BDNF Level

    Time frame: Baseline- immediately after exercise and 1 hours after exercise

    BDNF concentration will be determined from a saliva sample using the ELISA technique.

Secondary outcomes

  1. Saliva Flow Rate

    Time frame: Baseline- İmmediately after exercise and 1 hour after exercise

    It will be calculated individually by dividing the weight of the saliva collected within one minute by the duration.

Study contacts

Contact information is provided by the study sponsor or research team.

Cemre Didem Eyipınar, PhD

CONTACT

[email protected]

+90 545 264 54 86

Sponsors and collaborators

Lead sponsor

Cemre Didem Eyipinar

Other

Registry information

Official study title

The Effect of Aerobic Exercise on the Irisin/Klotho/Sirtuin-1/BDNF Axis: A Randomized Controlled Study

Important dates

Study start
2026
Primary completion
2027
Study completion
2027
First posted
Sep 2, 2026
Registry last updated
Sep 2, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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