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NCT Number: NCT07782736

Advanced Platelet-Rich Fibrin Plus in Nonsurgical Periodontal Therapy

The purpose of this randomized, controlled, split-mouth clinical trial was to determine whether adding advanced platelet-rich fibrin plus (A-PRF+), an autologous fibrin membrane prepared from the participant's own blood, to routine nonsurgical periodontal therapy could improve the healing of periodontal pockets.

Adults with periodontitis and periodontal sites with a probing pocket depth of 5 mm or greater and bleeding on probing were enrolled. For each participant, one side of the mouth was randomly assigned to receive scaling and root planing plus A-PRF+ membrane placement, while the other side received scaling and root planing alone. Periodontal probing depth, clinical attachment level, gingival recession, gingival index, bleeding on probing, and plaque index were assessed at baseline and after 4 and 8 weeks.

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Key information

Conditions

Periodontitis Mouth Diseases Periodontal Diseases Stomatognathic Diseases

Age range

18 year and older

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

Specialty Dental Clinic, Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City

Ho Chi Minh City, Vietnam

About this study

This single-center study used a randomized split-mouth design, allowing each participant to serve as their own control. Each participant drew a random assignment to determine the intervention and control sides of the mouth.

Both sides received routine nonsurgical periodontal therapy consisting of scaling and root planing. On the intervention side, A-PRF+ membranes prepared from autologous venous blood without anticoagulants or additives were additionally placed in eligible periodontal pockets. The control side received scaling and root planing without A-PRF+ placement.

Clinical periodontal parameters were recorded at baseline, 4 weeks, and 8 weeks. The study evaluated the short-term adjunctive clinical effect of A-PRF+ on periodontal pocket healing compared with nonsurgical periodontal therapy alone.

Who can participate

Healthy volunteers accepted: No

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Participant-level criteria:

  • Adults aged 18 years or older.
  • Diagnosis of periodontitis according to the 2017 World Workshop criteria.
  • At least one eligible periodontal tooth/site in each half-mouth.
  • Provision of written informed consent and agreement to attend the scheduled follow-up visits.

Site-level criteria:

  • Probing pocket depth of 5 mm or greater at baseline.
  • Clinical attachment loss of 3 mm or greater at baseline.
  • Positive bleeding on probing at baseline.

Exclusion criteria

Participant-level criteria:

  • Current use of tobacco or related products.
  • Systemic conditions closely associated with periodontitis or treatment response, including diabetes mellitus, rheumatoid arthritis, or hematologic disease.
  • Pregnancy or lactation.
  • Periodontal surgery within the preceding 6 months.
  • Use of medications affecting blood coagulation.
  • Abnormal preintervention complete blood count, prothrombin time, or activated partial thromboplastin time.
  • Poor oral hygiene.
  • Failure to attend protocol-specified visits.

Tooth/site-level criteria:

  • Untreated dental caries or pulpal disease.
  • Teeth undergoing active orthodontic treatment.
  • Teeth with defective restorations.
  • Third molars.
  • Teeth with mobility grade II or greater.
  • Sites with a plaque index of 2 or greater during follow-up were excluded from the site-level analysis.

Treatment and study plan

Scaling and Root Planing

Procedure

Nonsurgical mechanical periodontal treatment was performed to remove supra- and subgingival plaque and calculus and to debride the root surfaces.

Advanced Platelet-Rich Fibrin Plus

Biological

The autologous A-PRF+ membrane was prepared from venous blood collected without anticoagulants or additives and centrifuged at 1,300 rpm for 8 minutes. Following mechanical debridement and routine pocket irrigation, intervention pockets were rinsed with sterile saline before A-PRF+ membrane placement. The membrane was placed into eligible periodontal pockets and stabilized using an adhesive periodontal patch.

Primary outcomes

  1. Probing Pocket Depth

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Probing pocket depth was measured in millimeters using a periodontal probe, from the gingival margin to the base of the periodontal pocket. Lower values indicate shallower periodontal pockets.

  2. Clinical Attachment Level

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Clinical attachment level was measured in millimeters using a periodontal probe, from the cementoenamel junction to the base of the periodontal pocket. Lower values indicate less clinical attachment loss.

Secondary outcomes

  1. Gingival Recession

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Gingival recession was measured in millimeters from the cementoenamel junction to the gingival margin using a periodontal probe.

  2. Gingival Index

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Gingival inflammation was assessed at each periodontal site using an ordinal gingival index scored from 0 to 3, with higher scores indicating greater gingival inflammation.

  3. Bleeding on Probing

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Description: Bleeding on probing was recorded as present or absent after periodontal probing and reported as the number and percentage of sites with a positive bleeding response.

  4. Plaque Index

    Time frame: Baseline, 4 weeks, and 8 weeks after treatment

    Plaque accumulation at each periodontal site was assessed using an ordinal plaque index scored from 0 to 3, with higher scores indicating greater plaque accumulation.

Sponsors and collaborators

Lead sponsor

University of Medicine and Pharmacy at Ho Chi Minh City

Other

Registry information

Official study title

Application of Advanced Platelet-Rich Fibrin Plus (A-PRF+) in Nonsurgical Periodontal Therapy

Important dates

Study start
2025
Primary completion
2026
Study completion
2026
First posted
Aug 24, 2026
Registry last updated
Aug 24, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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