The Use of Advanced Platelet-rich Fibrin in Mandibular Third Molar Surgery

The purpose of the study is to quantify the levels of platelets and inflammatory markers in A-PRF from a standard venous whole blood sample, and to evaluate the effect of A-PRF on postoperative discomfort and complications following surgical removal of M3.

The study will be prospective and include 60 patients referred to Specialtandlægerne Vestergade for surgical removal of one M3. It is expected that the study will contribute with important information on the impact of A-PRF on postoperative sequelae and complications following surgical removal of mandibular third molars.

Completed

Looking for future studies?

Notify Me

Key information

Conditions

Third Molars Extraction

Age range

18 year and older

Sex eligibility

All sexes

Study type

Observational

Primary location

University of Copenhagen

Copenhagen, Denmark

About this study

Three venous whole blood samples will be collected by a dental assistant, which has undergone special training, supervised by a medical doctor affiliated with Specialtandlægerne Vestergade.

Surgical removal of M3 will be performed by one certified maxillofacial surgeon under local anaesthesia using a standard technique.

An incision from the anterior border of the ascending ramus of the mandible to the distal part of the lower first molar will be performed. The muco-periosteal flap will be reflected and the bone around the M3 will be removed with a round burr under constant irrigation with 0.9% saline solution. If necessary, the M3 will be sectioned with a fissure bur before the tooth is elevated out. The extraction socket and surrounding bone will be irrigated with 0.9 % saline solution, and the surgical site will be sutured with vicryl suture.

All patients will receive standard postoperative instructions. Sutures will be removed 7 days postoperatively. All patients will be seen in the clinic before surgery (T0) and one week following surgical removal of M3 .

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

indication for surgical removal of mandibular third molar

Exclusion criteria

breastfeading, pregnancy

Treatment and study plan

Third molar

Procedure

Evaluate the level of thrombocytes and growth factors in A-PRF and whole blood sample

Primary outcomes

Levels of thrombocytes and growth factors in advanced platelet-rich fibrin

Time frame: From enrollment to one week following surgery

Quantification of thrombocytes and specific growth factors (FGF, TGH-B1, VEGF, PDGF-B) released from advanced platelet-rich fibrin measured in concentration levels

Sponsors and collaborators

Lead sponsor

University of Copenhagen

Other

Registry information

Official study title

The Use of Advanced Platelet-rich Fibrin in Mandibular Third Molar Surgery. Levels of Platelets, Leucocytes, Inflammatory Biomarkers and the Effect on Postoperative Discomfort and Complications.

Important dates

Study start: 2024
Primary completion: 2024
Study completion: 2025
First posted: Aug 12, 2026
Registry last updated: Aug 12, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

Published trials that share one or more normalized conditions with this study.

Completed