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OpenTrials
Completed

NCT Number: NCT07760025

The Effect of Culture Media Visualization-Supported Hand Hygiene Education on Handwashing Behavior in Preschool Children

The hand hygiene educational intervention supported by culture medium visualization was found to be an effective practice in promoting proper handwashing behaviors and reducing hand microbial load in children aged 5-6 years. The educational intervention featuring culture plate demonstrations applied to the intervention group was determined to be highly effective in achieving hand hygiene and reducing hand bacterial load. In contrast, the control group received traditional education, which did not yield a statistically significant difference.

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Key information

Age range

5 year–6 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Primary location

A Preschool

Ankara, 06018, Turkey (Türkiye)

About this study

Objective: The study aims to evaluate the effectiveness of a culture medium visualization supported hand hygiene education program implemented for preschool children on their acquisition of handwashing skills and habits.

Materials and Method: The study was conducted using a pretest-postted randomized controlled experimental design, one of the quantitave research methods. The research was carried out with 59 preschool children aged 5-6 years in a kindergarten located in the Keçiören district of Ankara Province. Handwashing behaviors of the children were observed before and after the intervention and recorded using the Handwashing Behavior Assessment Form. To evaluate hand microbial load, hand culture samples were collected using biplate 5% Sheep Blood+Chromogenic UTI Agar. For data analysis, the Chi-Square test was used to compare categorical variables and the McNemar test was performed to evaluate the comparative effectiveness of culture medium visualization before and after the training. An ANCOVA test was applied to assess baseline measurements of continuous variables between groups and to evaluate the effect of the educational intervention.

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Parental consent obtained.
  • The child whose parental consent was obtained is willing to participate in the study.
  • Children aged 4-6 years attending A Preschool.
  • Children without any cognitive or developmental disabilities.
  • Children without hearing or visual impairments.
  • Children who can speak and understand Turkish.

Exclusion criteria

  • Absence of parental consent.
  • Children who were unwilling to participate in the study despite parental consent being obtained.
  • Children with skin conditions or skin allergies.

Treatment and study plan

Hand hygiene, handwashing behavior intervention

Behavioral

Agar plates were shown to the intervention group, allowing the demonstration of bacterial colonies.

Standard Hand Hygiene Education

Behavioral

Participants in this group received standard hand hygiene education without culture media visualization using agar plates.

Primary outcomes

  1. Handwashing Behavior

    Time frame: Baseline and approximately 6 weeks after the intervention

    Handwashing behavior was assessed by direct observation using a structured handwashing behavior observation form before and after the educational intervention.

Sponsors and collaborators

Lead sponsor

Esra Şengün Deniz

Other

Collaborators

  • Ministry of National Education, Turkey

Registry information

Important dates

Study start
2026
Primary completion
2026
Study completion
2026
First posted
Aug 12, 2026
Registry last updated
Aug 12, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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