Fluorescent Hand Hygiene Training and Long-Term Effects in First-Year Nursing Students

This single-group quasi-experimental study will evaluate the effectiveness and durability of a fluorescent hand hygiene training program for first-year nursing students at a Polish university. Students will complete the WHO Hand Hygiene Knowledge Questionnaire and undergo Glo Germ-based fluorescent assessment of handwashing technique at baseline, immediately after the training, approximately two weeks later, at the end of the first semester, and just before their first clinical placements. The primary objective is to determine whether the training improves and sustains hand hygiene technique quality over time, while secondary objectives include changes in hand hygiene knowledge and the relationship between knowledge and technique

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Key information

Conditions

Hand Hygiene Compliance Hand Hygiene Hand Hygiene Behavior Hand Hygiene Effectiveness

Age range

18 year–55 year

Sex eligibility

All sexes

Study type

Interventional

Phase

Not applicable

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

First-year nursing students enrolled at Powiślańska Academy of Applied Sciences in Kwidzyn during the study period.
Aged 18 years or older.
Able to provide written informed consent.
Able to participate in the planned educational activities.

Exclusion criteria

Known allergy or skin intolerance to Glo Germ™ /gel or similar products.
Current dermatological condition of the hands that precludes the use of fluorescent lotion/gel or repeated handwashing (e.g., severe eczema or open wounds).
Previous participation in a pilot study or an earlier version of the same fluorescent hand-hygiene training at this institution.

Treatment and study plan

Fluorescent Hand Hygiene Training with Glo Germ

Behavioral

First-year nursing students receive a standardized fluorescent hand hygiene training session using Glo Germ lotion and UV light. The session includes a brief review of WHO hand hygiene indications, demonstration of proper handwashing technique, application of fluorescent lotion, handwashing by the student, and visual feedback of missed areas under UV illumination. Students repeat handwashing until adequate coverage is achieved. This training is delivered early in the first semester and its effects are assessed at immediate, short-term, semester-end, and pre-clinical time points

Primary outcomes

Quality of hand hygiene technique assessed with fluorescent Glo Germ

Time frame: Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements
Quality of hand hygiene technique assessed with fluorescent Glo Germ

Time frame: Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements

Hand hygiene technique will be evaluated at five time points using Glo Germ fluorescent lotion and UV light. After applying fluorescent lotion, students wash their hands as usual; residual fluorescence indicating missed areas is recorded by the investigator on a standardized checklist (e.g., fingertips, thumbs, interdigital spaces, dorsal surfaces). The primary outcome will be the proportion of students achieving adequate technique (no or minimal missed areas) and changes in individual technique scores over time

Sponsors and collaborators

Lead sponsor

Powiślańska Academy of Applied Sciences

Other

Registry information

Official study title

Durability of Fluorescent Hand Hygiene Training Effects Among First-Year Nursing Students: A Single-Group Quasi-Experimental Before-After Study With Repeated Measures of Knowledge and Technique

Important dates

Study start: 2026
Primary completion: 2027
Study completion: 2027
First posted: Aug 11, 2026
Registry last updated: Aug 11, 2026

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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