Impact of Microbiome-changing Interventions on Food Decision-making

BOLD-acitvity will be measured using event-related echo-planar T2*-weighted magnetic resonance imaging (MRI) during food wanting task according to previously described procedures (details of preprocessing at: https://osf.io/ynkxw). Onsets of food and art stimuli presentation will be modelled as separate regressors convolving delta functions with a canonical hemodynamic response function. Wanting rating scores (on an 8-point likert scale) per stimulus will be added as covariates. In a parallel model, kcal of food stimuli will be added multiplied with wanting scores to model high caloric food wanting interaction. At the group level, we will assess the contrasts food > art and wanting modulation.

Changes in alpha and beta diversity assessed using 16S rRNA sequencing of stool samples

BOLD-acitvity will be measured using event-related echo-planar T2*-weighted magnetic resonance imaging (MRI) during food wanting and food memory recognition tasks according to previously described procedures (details of preprocessing at: https://osf.io/ynkxw). Onsets of food and art stimuli presentation in encoding and recognition will be modelled as separate regressors convolving delta functions with a canonical hemodynamic response function. Correctly remembered and correctly rejected, as well as misses and false alarms will be modeled separately. In parallel models, similar and new items als well as wanting rating scores (on an 8-point likert scale) per stimulus and kcal of food stimuli multiplied with wanting scores will be added as covariates. At the group level, we will assess the contrasts correct > false and wanting modulation.

self-reported hunger feeling on a 8-point score from minimum 0 (not at all) to maximum 8 (very much).

ghrelin pg/ml in blood

leptin ng/ml in blood

Glucagon-like peptide-1 (GLP-1) pg/ml in blood

peptide YY pg/ml in blood

insulin in blood uU/ml

hemoglycated globulin A1c in blood %

high sensitive C-reactive protein mg/l (and optionally tumor necrosis factor alpha and interleukin 6 in blood, pg/ml)

short-chain fatty acids (SCFA), bile acids

weight kg/height in m squared

waist (cm) to hip (cm) circumeference ratio

body fat % according to bioelectrical impedance analysis

assessed with the food craving questionnaire (Meule et al., 2012, German version).

measured using the attention network task (ANT)