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NCT Number: NCT03925311

Transcriptome Profiling and Endometrial Receptivity

The aim of this study is to correlate and identify the biochemical markers which may reflect WOI. Blood samples will be collected and endometrial biopsy will be performed at particular time points.

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Key information

Conditions

Pregnancy Related

Age range

20 year–40 year

Sex eligibility

Female

Study type

Observational

Primary location

Chinese University of Hong Kong

Hong Kong

Location status: Recruiting

About this study

Endometrial receptivity in the midluteal phase of the menstrual cycle, known as the window of implantation (WOI), occurs only for a very short period of time. However, so far, there is no reliable clinical and biochemical indicators of WOI. Therefore, this study is to correlate and identify the biochemical markers which may reflect WOI.

participants will be asked to be:

  • performed endometrial biopsy
  • taken blood for a few time points (e.g. pre-conception and being pregnant) and to compare different kinds of biochemical markers.

Who can participate

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

Age 20-40 years.

Exclusion criteria

  • Uncorrected uterine anomalies, such as septate or bicornuate uterus, fibroids (submucous/intramural).
  • Intrauterine adhesions.
  • Adenomyosis.

Treatment and study plan

endometrial biopsy and blood sampling

Procedure

patient will be performed endometrial biopsy and collected blood samples at particular time points

Primary outcomes

  1. The change of the transcriptome profiling in the peripheral blood

    Time frame: From the day of endometrial biopsy through study completion, an average of 2 months

    RNA per sample will be prepared into two portions. One is for RNA sequencing and the other is for small RNA sequencing. The next step is hydrolysis of RNA into 200-300 nucleotides prior to reverse transcription. When sequencing RNA other than mRNA the library preparation is modified. The cellular RNA is selected based on the desired size range. For miRNA, the RNA is isolated through size selection. Once isolated, linkers are added to the 3' and 5' end then purified. The final step is cDNA generation through reverse transcription.

  2. The change of the immune markers in the peripheral blood

    Time frame: From the day of endometrial biopsy through study completion, an average of 2 months

    Immune markers including cytokines such as leukemia inhibitory factor, macrophage colony stimulating factor, interleukin - 1 beta, transforming growth factor beta-1, Tim-3, galectin-9 and other markers considered to play a role in implantation will also be measured.

Interested in participating?

Recruiting

Interested in participating?

Request Info

Sponsors and collaborators

Lead sponsor

Chinese University of Hong Kong

Other

Registry information

Official study title

Transcriptome Profiling and Immune/ Biochemical Markers of Peripheral Blood to Predict Endometrial Receptivity and Successful Implantation

Important dates

Study start
2015
Primary completion
2025
Study completion
2025
First posted
Apr 24, 2019
Registry last updated
Mar 27, 2025

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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