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Completed

NCT Number: NCT02801344

The Impact of Protein Intake on Protein Metabolism During Intensified Training

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake.

The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.

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Key information

Conditions

Healthy Behavior Motor Activity

Age range

18 year–50 year

Sex eligibility

Male

Study type

Interventional

Phase

Not applicable

Primary location

Goldring Centre For High Performance Sport

Toronto, Ontario, M5S 2C9, Canada

Who can participate

Healthy volunteers accepted: Yes

Only the study team can determine whether someone qualifies for participation.

Inclusion criteria

  • Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week
  • Participants who are categorized at least "very good" based on a study by Shvartz & Reibold [40], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age,
  • Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2.
  • Participants will be 18-50 years old.
  • Participants are willing to abide by the compliance rules of this study

Exclusion criteria

  • Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone.
  • Female
  • Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor
  • Regular tobacco use
  • Illicit drug use (e.g. growth hormone, testosterone, etc.)

Treatment and study plan

Normal

Dietary Supplement

test drink which contains low amount of amino acids (0.14 g/kg/day)

Moderate

Dietary Supplement

test drink which contains moderate amount of amino acids (0.40 g/kg/day)

High

Dietary Supplement

test drink which contains high amount of amino acids (1.03 g/kg/day)

Controlled-diet

Other

the diet containing 0.8 g protein /kg/day

Primary outcomes

  1. Change in 24h whole body protein balance

    Time frame: Difference between day 1 and day 4 whole body protein balance

    Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using [15N]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels

Secondary outcomes

  1. Change from baseline physical performance (5 km time trial) 5days after the beginning of training

    Time frame: 5days after the beginning of training

    Before and after 4 days of intensified training period, Duration (seconds) for completing 5-km running on treadmill will be measured.

  2. change from baseline Physical performance (muscle strength) 5days after the beginning of training

    Time frame: 5days after the beginning of training

    Maximal strength will be assessed by a maximal isometric voluntary contraction (MVC), which will be tested for the knee-extensors using a custom-made knee-extension apparatus after 4 days intensified training.

  3. change from baseline Physical Performance (muscle power) 5days after the beginning of training

    Time frame: 5days after the beginning of training

    Peak lower body power will be measured by force platform after 4days-intensified training.

Other outcomes

  1. Creatine Phosphate Kinase activity on day 5

    Time frame: 5days after the beginning of training

    Muscle damage markers (blood Creatine Phosphate Kinase activity, IU/L) will be compared between the experimental arms 5days after the beginning of training

  2. Myoglobin concentration on day 5

    Time frame: 5days after the beginning of training

    Muscle damage markers (blood Mb Concentration, mg/mL) will be compared between the experimental arms 5days after the beginning of training

  3. IL-6 concentration on day 5

    Time frame: 5days after the beginning of training

    inflammatory markers (IL-6 concentration, pg/ml) will be compared between the experimental arms 5days after the beginning of training

  4. CRP concentration on day 5

    Time frame: 5days after the beginning of training

    inflammatory markers (CRP concentration, mg/L) will be compared between the experimental arms 5days after the beginning of training

  5. amino acid concentration on day 5

    Time frame: 5days after the beginning of training

    amino acid concentration (mmol/mL) will be compared between the experimental arms 5days after the beginning of training

Sponsors and collaborators

Lead sponsor

University of Toronto

Other

Collaborators

  • Ajinomoto Co., Inc.

Registry information

Official study title

The Impact of Protein Intake on Amino Acid and Protein Metabolism, and Performance During Intensified Training

Important dates

Study start
2016
Primary completion
2017
Study completion
2018
First posted
Jun 15, 2016
Registry last updated
Mar 29, 2019

OpenTrials presents study information sourced from ClinicalTrials.gov. The official registry record should be consulted for the latest information.

View the official ClinicalTrials.gov record (opens in a new tab)

This listing is for discovery and informational purposes only. It is not medical advice, does not guarantee that a study is recruiting, and does not determine eligibility. Contact the study team and a qualified healthcare professional when considering participation.

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